Proteintech, 14270-1-AP, ITPKA Polyclonal antibody

Size: 20ul / 150ul The ITPKA (14270-1-AP) by Proteintech is a Polyclonal antibody targeting ITPKA in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14270-1-AP targets ITPKA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3200 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information ITPKA(Inositol-trisphosphate 3-kinase A) belongs to the inositol phosphokinase (IPK) family. It accumulates in dendritic spines and seems to be critically involved in synaptic plasticity. The protein possesses two functional activities. In neurons, ITPKA is targeted to F-actin via an N-terminal actin binding domain (amino acids 1-66) and is relevant for long term potentiation and spatial learning(PMID:20022963). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5588 Product name: Recombinant human ITPKA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 141-461 aa of BC026331 Sequence: LLSDSESRSRGNVQLEAGEDVGQKNHWQKIRTMVNLPVISPFKKRYAWVQLAGHTGSFKAAGTSGLILKRCSEPERYCLARLMADALRGCVPAFHGVVERDGESYLQLQDLLDGFDGPCVLDCKMGVRTYLEEELTKARERPKLRKDMYKKMLAVDPEAPTEEEHAQRAVTKPRYMQWREGISSSTTLGFRIEGIKKADGSCSTDFKTTRSREQVLRVFEEFVQGDEEVLRRYLNRLQQIRDTLEVSEFFRRHEVIGSSLLFVHDHCHRAGVWLIDFGKTTPLPDGQILDHRRPWEEGNREDGYLLGLDNLIGILASLAER Predict reactive species Full Name: inositol 1,4,5-trisphosphate 3-kinase A Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 51 kDa GenBank Accession Number: BC026331 Gene Symbol: ITPKA Gene ID (NCBI): 3706 RRID: AB_2129841 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P23677 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.