Proteintech, 14292-1-AP, CD80 Polyclonal antibody

Size: 20ul / 150ul The CD80 (14292-1-AP) by Proteintech is a Polyclonal antibody targeting CD80 in WB, ELISA applications with reactivity to human, mouse samples 14292-1-AP targets CD80 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Raji cells, RAW 264.7 cells, human peripheral blood leukocyte, Ramos cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5615 Product name: Recombinant human B7-1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 36-228 aa of BC042665 Sequence: IHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQT Predict reactive species Full Name: CD80 molecule Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC042665 Gene Symbol: CD80 Gene ID (NCBI): 941 RRID: AB_10640809 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P33681 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.