Proteintech, 14336-1-AP, HLX Polyclonal antibody

Size: 20ul / 150ul The HLX (14336-1-AP) by Proteintech is a Polyclonal antibody targeting HLX in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 14336-1-AP targets HLX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human placenta tissue Positive IHC detected in: human endometrial cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information In immune system, Hlx is a Th1-special transcription factor that interacts specifically with T-bet, and synergistically they promote IFN-γ expression when co-expressed. It can down-regulate the IL-4 receptor expression in naive CD4+ T cells [PMID:14688316]. Over expression of Hlx contributes ot the aberrant expression of IFN-γ in normal CD4+ T cells during the differentiation under Th2-polarizing conditions. In addition, Hlx is a negative regulator of IFN-γ production in NK cells and its inhibitory function is achieved at least in part through the proteasomal degradation of STAT4 [PMID:16424184]. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5627 Product name: Recombinant human HLX protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 134-488 aa of BC033808 Sequence: QQQQPPPPPRAGALQPPASGTRVVPNPHHSGSAPAPSSKDLKFGIDRILSAEFDPKVKEGNTLRDLTSLLTGGRPAGVHLSGLQPSAGQFFASLDPINEASAILSPLNSNPRNSVQHQFQDTFPGPYAVLTKDTMPQTYKRKRSWSRAVFSNLQRKGLEKRFEIQKYVTKPDRKQLAAMLGLTDAQVKVWFQNRRMKWRHSKEAQAQKDKDKEAGEKPSGGAPAADGEQDERSPSRSEGEAESESSDSESLDMAPSDTERTEGSERSLHQTTVIKAPVTGALITASSAGSGGSSGGGGNSFSFSSASSLSSSSTSAGCASSLGGGGASELLPATQPTASSAPKSPEPAQGALGCL Predict reactive species Full Name: H2.0-like homeobox Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 51 kDa GenBank Accession Number: BC033808 Gene Symbol: HLX Gene ID (NCBI): 3142 RRID: AB_2232971 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14774 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.