Proteintech, 14380-1-AP, NIT1 Polyclonal antibody

Size: 20ul / 150ul The NIT1 (14380-1-AP) by Proteintech is a Polyclonal antibody targeting NIT1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 14380-1-AP targets NIT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, HeLa cells, HEK-293T cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5764 Product name: Recombinant human NIT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-243 aa of BC046149 Sequence: MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGPCLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEPVSS Predict reactive species Full Name: nitrilase 1 Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 32-36 kDa GenBank Accession Number: BC046149 Gene Symbol: NIT1 Gene ID (NCBI): 4817 RRID: AB_2151314 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86X76 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.