Proteintech, 14412-1-AP, PEG10 Polyclonal antibody

Size: 20ul / 150ul The PEG10 (14412-1-AP) by Proteintech is a Polyclonal antibody targeting PEG10 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 14412-1-AP targets PEG10 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, L02 cells, MCF-7 cells, MDA-MB-231 cells, T-47D cells Positive IHC detected in: mouse skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Paternally expressed imprinted gene 10 (PEG10) is an evolutionarily conserved HERV gene belonging to the Ty3/Gypsy family of retrotransposons. The mRNA of PEG10 encodes at least two protein isoforms: the major PEG10 protein product reading frame 1 (RF1-PEG10, ~50 kDa) and reading frames 1 and 2 (RF1/RF2-PEG10, ~100 kDa), which are translated by a typical retroviral-1 ribosomal frameshift mechanism. PEG10 is strongly expressed in placenta, ovary and testis as well as somatic adult organs including brain, kidney and lung . Researches on mice suggest that PEG10 plays an important role in placenta formation and adipocyte differentiation, and its knockout can cause embryonic lethality.(PMID: 30123090, PMID: 32244497) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5754 Product name: Recombinant human PEG10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC050659 Sequence: MTERRRDELSEEINNLREKVMKQSEENNNLQSQVQKLTEENTTLREQVEPTPEDEDDDIELRGAAAAAAPPPPIEEECPEDLPEKFDGNPDMLAPFMAQCQIFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMMEMKHVFEDPQRREVAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQEELSHLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALVLPHIASHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGTGGHYADNCPAKASKSSPAGNSPAPL Predict reactive species Full Name: paternally expressed 10 Calculated Molecular Weight: 80 kDa Observed Molecular Weight: 50 kDa, 100 kDa GenBank Accession Number: BC050659 Gene Symbol: PEG10 Gene ID (NCBI): 23089 RRID: AB_10694427 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86TG7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.