Proteintech, 14423-1-AP, RBM10 Polyclonal antibody

Size: 20ul / 150ul The RBM10 (14423-1-AP) by Proteintech is a Polyclonal antibody targeting RBM10 in WB, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 14423-1-AP targets RBM10 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, human brain tissue, mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) . Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5788 Product name: Recombinant human RBM10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 601-930 aa of BC024153 Sequence: QSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAKDMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAGYAILEKKGALAERQHTSMDLPKLASDDRPSPPRGLVAAYSGESDSEEEQERGGPEREEKLTDWQKLACLLCRRQFPSKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDMEQMKYRDRAAERREKYGIPEPPEPKRRKYGGISTASVDFEQPTRDGLGSDNIGSRMLQAMGWKEGSGLGRKKQGIVTPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMVTRFNEAQ Predict reactive species Full Name: RNA binding motif protein 10 Calculated Molecular Weight: 104 kDa Observed Molecular Weight: 115 kDa GenBank Accession Number: BC024153 Gene Symbol: RBM10 Gene ID (NCBI): 8241 RRID: AB_2175593 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P98175 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.