Proteintech, 14475-1-AP, GSTA1 Polyclonal antibody

Size: 20ul / 150ul The GSTA1 (14475-1-AP) by Proteintech is a Polyclonal antibody targeting GSTA1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14475-1-AP targets GSTA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, human kidney tissue, HepG2 cells, mouse liver tissue, rat kidney tissue, rat liver tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human lung cancer tissue, human liver tissue, human colon cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information GSTA1 encodes one of the GST alpha isozymes, belongs to GST family, which is mainly expressed in the liver. GST can improve the detoxification of organism by enzymatic and non-enzymatic methods, and has anti-mutagenic and anti-tumor effects. Studies have shown that overexpression of GSTA1 can promote the development of lung cancer cells, so the expression level of GSTA1 can be used to predict the degree of malignancy of lung cancer tissues. To some extent, GSTA1 is regarded as a potential lung cancer candidate targets. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5849 Product name: Recombinant human GSTA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC053578 Sequence: MAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIADLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRKPPMDEKSLEEARKIFRF Predict reactive species Full Name: glutathione S-transferase alpha 1 Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: BC053578 Gene Symbol: GSTA1 Gene ID (NCBI): 2938 RRID: AB_2232810 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P08263 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.