Proteintech, 14595-1-AP, TERF2IP Polyclonal antibody

Size: 20ul / 150ul The TERF2IP (14595-1-AP) by Proteintech is a Polyclonal antibody targeting TERF2IP in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 14595-1-AP targets TERF2IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, A431 cells, HeLa cells, HT-1080 cells, human placenta tissue, K-562 cells Positive IP detected in: HeLa cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6130 Product name: Recombinant human TERF2IP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-268 aa of BC005841 Sequence: MAEAMDLGKDPNGPTHSSTLFVRDDGSSMSFYVRPSPAKRRLSTLILHGGGTVCRVQEPGAVLLAQPGEALAEASGDFISTQYILDCVERNERLELEAYRLGPASAADTGSEAKPGALAEGAAEPEPQRHAGRIAFTDADDVAILTYVKENARSPSSVTGNALWKAMEKSSLTQHSWQSLKDRYLKHLRGQEHKYLLGDAPVSPSSQKLKRKAEEDPEAADSGEPQNKRTPDLPEEEYVKEEIQENEEAVKKMLVEATREFEEVVVDE Predict reactive species Full Name: telomeric repeat binding factor 2, interacting protein Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC005841 Gene Symbol: TERF2IP Gene ID (NCBI): 54386 RRID: AB_10642697 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NYB0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.