Proteintech, 14716-1-AP, KLF6 Polyclonal antibody

Size: 20ul / 150ul The KLF6 (14716-1-AP) by Proteintech is a Polyclonal antibody targeting KLF6 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 14716-1-AP targets KLF6 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse thymus tissue, mouse colon tissue, HUVEC cells, NCI-H1299 cells Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 has 3 isoforms with the molecular mass of 32, 31 and 26kDa. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa), Highly ubiquitinated KLF6(~60kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6440 Product name: Recombinant human KLF6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 6-283 aa of BC000311 Sequence: MCSIFQELQIVHETGYFSALPSLEEYWQQTCLELERYLQSEPCYVSASEIKFDSQEDLWTKIILAREKKEESELKISSSPPEDTLISPSFCYNLETNSLNSDVSSESSDSSEELSPTAKFTSDPIGEVLVSSGKLSSSVTSTPPSSPELSREPSQLWGCVPGELPSPGKVRSGTSGKPGDKGNGDASPDGRRRVHRCHFNGCRKVYTKSSHLKAHQRTHTGEKPYRCSWEGCEWRFARSDELTRHFRKHTGAKPFKCSHCDRCFSRSDHLALHMKRHL Predict reactive species Full Name: Kruppel-like factor 6 Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 32-42 kDa GenBank Accession Number: BC000311 Gene Symbol: KLF6 Gene ID (NCBI): 1316 RRID: AB_10640526 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99612 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.