Proteintech, 14743-1-AP, DRG2 Polyclonal antibody

Size: 20ul / 150ul The DRG2 (14743-1-AP) by Proteintech is a Polyclonal antibody targeting DRG2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14743-1-AP targets DRG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, mouse brain tissue, SMMC-7721 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human kidney tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:3000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. DRG2 plays a critical role in control of the cell cycle and apoptosis (PMID:27669826, PMID:14759600). DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6433 Product name: Recombinant human DRG2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-364 aa of BC000493 Sequence: MGILEKISEIEKEIARTQKNKATEYHLGLLKAKLAKYRAQLLEPSKSASSKGEGFDVMKSGDARVALIGFPSVGKSTFLSLMTSTASEAASYEFTTLTCIPGVIEYKGANIQLLDLPGIIEGAAQGKGRGRQVIAVARTADVIIMMLDATKGEVQRSLLEKELESVGIRLNKHKPNIYFKPKKGGGISFNSTVMLTQCSEKLVQLILHEYKIFNAEVLFREDCSPDEFIDVIVGNRVYMPCLYVYNKIDQISMEEVDRLARKPNSVVISCGMKLNLDYLLEMLWEYLALTCIYTKKRGQRPDFTDAIILRKGASVEHVCHRIHRSLASQFKYALVWGTSTKYSPQRVGLTHTMEHEDVIQIVKK Predict reactive species Full Name: developmentally regulated GTP binding protein 2 Calculated Molecular Weight: 41 kDa Observed Molecular Weight: 41-45 kDa GenBank Accession Number: BC000493 Gene Symbol: DRG2 Gene ID (NCBI): 1819 RRID: AB_2230800 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P55039 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.