Proteintech, 14783-1-AP, USP36 Polyclonal antibody

Size: 20ul / 150ul The USP36 (14783-1-AP) by Proteintech is a Polyclonal antibody targeting USP36 in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 14783-1-AP targets USP36 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells Positive IP detected in: Jurkat cells Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6411 Product name: Recombinant human USP36 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC038983 Sequence: MPIVDKLKEALKPGRKDSADDGELGKLLASSAKKVLLQKIEFEPASKSFSYQLEALKSKYVLLNPKTEGASRHKSGDDPPARRQGSEHTYESCGDGVPAPQKVLFPTERLSLRWERVFRVGAGLHNLGNTCFLNATIQCLTYTPPLANYLLSKEHARSCHQGSFCMLCVMQNHIVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGCAKCVLPHPPSQGFGHLFYMVAAEHSYGNSQPGTFLPRLPLGRRGRICLWVLKLLEPK Predict reactive species Full Name: ubiquitin specific peptidase 36 Calculated Molecular Weight: 1123 aa, 123 kDa Observed Molecular Weight: 100-150 kDa GenBank Accession Number: BC038983 Gene Symbol: USP36 Gene ID (NCBI): 57602 RRID: AB_2213357 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P275 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.