Proteintech, 14799-1-AP, RPS4X Polyclonal antibody

Size: 20ul / 150ul The RPS4X (14799-1-AP) by Proteintech is a Polyclonal antibody targeting RPS4X in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14799-1-AP targets RPS4X in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse pancreas tissue, mouse liver tissue, rat liver tissue Positive IP detected in: HepG2 cells Positive IHC detected in: human colon tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). [PMID:8358435] Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, xenopus, frog Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6513 Product name: Recombinant human SCAR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC000472 Sequence: MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG Predict reactive species Full Name: ribosomal protein S4, X-linked Calculated Molecular Weight: 263 aa, 30 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC000472 Gene Symbol: RPS4X Gene ID (NCBI): 6191 RRID: AB_2238567 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62701 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.