Proteintech, 14812-1-AP, ISCU Polyclonal antibody

Size: 20ul / 150ul The ISCU (14812-1-AP) by Proteintech is a Polyclonal antibody targeting ISCU in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14812-1-AP targets ISCU in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, Raji cells, A549 cells, HeLa cells, COLO 320 cells Positive IP detected in: mouse heart tissue Positive IHC detected in: human endometrial cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Iron-sulfur (Fe-S) clusters are required for the functions of mitochondrial aconitase, mammalian iron regulatory protein 1, and many other proteins in multiple subcellular compartments. Mitochondrial and cytosolic isoforms of the human Fe-S cluster scaffold protein, iron-sulfur cluster assembly enzyme (ISCU), is involved in activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins.Defects in ISCU gene are the cause of myopathy with exercise intolerance Swedish type (MEIS), which is characterized by lifelong severe exercise intolerance (PMID: 18304497). Two ISCU isoforms ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing, and ISCU2 is considered the canonical isoform1. ISCU2 protein is rapidly processed upon maturation (PMID: 11060020). Proteintech's ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14 kDa,15 kDa and 18-19 kDa bands which has been reported in the previous observation (PMID: 11060020). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6562 Product name: Recombinant human ISCU protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC061903 Sequence: MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK Predict reactive species Full Name: iron-sulfur cluster scaffold homolog (E. coli) Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 14-15 kDa, 18-19 kDa GenBank Accession Number: BC061903 Gene Symbol: ISCU Gene ID (NCBI): 23479 RRID: AB_2280362 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H1K1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.