Proteintech, 14834-1-AP, ATF2-Specific Polyclonal antibody

Size: 20ul / 150ul The ATF2-Specific (14834-1-AP) by Proteintech is a Polyclonal antibody targeting ATF2-Specific in WB, IHC, ELISA applications with reactivity to human, mouse samples 14834-1-AP targets ATF2-Specific in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, LNCaP cells, C2C12 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human ATF2. The calculated molecular weight of ATF2 is 55 kDa, but the modified ATF2 protein is about 70 kDa (PMID: 18269766 ). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: activating transcription factor 2 Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC026175 Gene Symbol: ATF2 Gene ID (NCBI): 1386 RRID: AB_10694139 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15336 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.