Proteintech, 14948-1-AP, LMOD3 Polyclonal antibody

Size: 20ul / 150ul The LMOD3 (14948-1-AP) by Proteintech is a Polyclonal antibody targeting LMOD3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 14948-1-AP targets LMOD3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information The gene encoding LMOD3 has not been characterized so far and very limited information of its function has been reported. Nanda et al. found that the expression of mouse LMOD3 mRNA is restricted largely to cardiac and skeletal muscle through RT-PCR analysis (PMID: 22157009). Two isoforms of LMOD3 may exist due to the alternative splicing, whose molecular weights are predicted as 65 kDa and 40 kDa, respectively (Uniprot). This antibody was raised against the N-terminal region of human LMOD3. It detects a double bands around 80 kDa and 65 kDa in heart and skeletal muscle lysates. The reason causing the discrepancy between the predicted and observed molecular weight is not clear. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, zebrafish, xenopus Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6758 Product name: Recombinant human LMOD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-236 aa of BC039202 Sequence: MSEHSRNSDQEELDEEINEDEILANLSAEELKELQSEMEVMAPDPSLPVGMIQKDQTDKPPTGNFNHKSLVDYMYWEKASRRMLEEERVPVTFVKSEEKTQEEHEEIEKRNKNMAQYLKEKLNNEIVANKRESKGSSNIQETDEEDEEEEDDDDDDEGEDDGEESEETNREEEGKAKEQIRNCENNCQQVTDKAFKEQRDRPEAQEKKKKKISQGKIIFRKNNVRAQQKFRSRRTR Predict reactive species Full Name: leiomodin 3 (fetal) Calculated Molecular Weight: 65 kDa Observed Molecular Weight: 65-70 kDa GenBank Accession Number: BC039202 Gene Symbol: LMOD3 Gene ID (NCBI): 56203 RRID: AB_2136690 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q0VAK6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.