Proteintech, 15089-1-AP, MBP-Tag Polyclonal antibody

Size: 150ul The MBP-Tag (15089-1-AP) by Proteintech is a Polyclonal antibody targeting MBP-Tag in WB, ELISA applications with reactivity to recombinant protein samples 15089-1-AP targets MBP-Tag in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: lane1: MBP-NOL6 64kd; lane2 MBP 42kd, Recombinant protein, transfected HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems. Specification Tested Reactivity: recombinant protein Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0942 Product name: Recombinant MBP-Tag protein Source: e coli. -derived, Unknow Tag: N/A Domain: pMAL of Sequence: Predict reactive species Full Name: MBP-Tag Calculated Molecular Weight: 40 kDa RRID: AB_1607774 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.