Proteintech, 15099-1-AP, DHDDS Polyclonal antibody

Size: 20ul / 150ul The DHDDS (15099-1-AP) by Proteintech is a Polyclonal antibody targeting DHDDS in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 15099-1-AP targets DHDDS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, Y79 cells Positive IHC detected in: mouse testis tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information DHDDS (dehydrodolichol diphosphate synthetase) encodes a protein involved in dolichol biosynthesis on the cytoplasmic face of the ER, where N-glycosylation, O-mannosylation, C-mannosylation and GPI-anchor synthesis occurs (PMID: 34382076). Homozygous mutations in the DHDDS gene (c.124 A>G) have been associated with nonsyndromic retinitis pigmentosa in consanguineous families (PMID: 36628425). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7426 Product name: Recombinant human DHDDS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC003643 Sequence: MSWIKEGELSLWERFCANIIKAGPMPKHIAFIMDGNRRYAKKCQVERQEGHSQGFNKLAETLRWCLNLGILEVTVYAFSIENFKRSKSEVDGLMDLARQKFSRLMEEKEKLQKHGVCIRVLGDLHLLPLDLQELIAQAVQATKNYNKCFLNVCFAYTSRHEISNAVREMAWGVEQGLLDPSDISESLLDKCLYTNRSPHPDILIRTSGEVRLSDFLLWQTSHSCLVFQPVLWPEYTFWNLFEAILQFQMNHSMLQKARDMYAEERKRQQLERDQATVTEQLLREGLQASGDAQLRRTRLHKLSARREERVQGFLQALELKRADWLARLGTASA Predict reactive species Full Name: dehydrodolichyl diphosphate synthase Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC003643 Gene Symbol: DHDDS Gene ID (NCBI): 79947 RRID: AB_2091576 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86SQ9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.