Proteintech, 15104-1-AP, TNIP1 Polyclonal antibody

Size: 20ul / 150ul The TNIP1 (15104-1-AP) by Proteintech is a Polyclonal antibody targeting TNIP1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 15104-1-AP targets TNIP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, mouse skeletal muscle tissue, HEK-293 cells, HeLa cells, U2OS cells Positive IP detected in: HeLa cells Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TNIP1, also named as KIAA0113, NAF1, Nip40-1, VAN and ABIN-1, is involved in inhibition of nuclear factor- B (NF- B) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3). TNIP1 repression of RARs in the presence of RA places it in a small category of corepressors of agonist-bound NRs. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1561 Product name: Recombinant human TNIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 287-636 aa of BC014008 Sequence: AGKVPEVVALGAAEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQKLADLQKQVTDLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASGERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNSRLFHLPEYTWRLPCGGVRNPNQSSQVMDPPTARPTEPESPKNDREGPQ Predict reactive species Full Name: TNFAIP3 interacting protein 1 Calculated Molecular Weight: 72 kDa Observed Molecular Weight: 72 kDa GenBank Accession Number: BC014008 Gene Symbol: TNIP1 Gene ID (NCBI): 10318 RRID: AB_2207757 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15025 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.