Proteintech, 15159-1-AP, MEN1 Polyclonal antibody

Size: 20ul / 150ul The MEN1 (15159-1-AP) by Proteintech is a Polyclonal antibody targeting MEN1 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15159-1-AP targets MEN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells Positive IP detected in: HEK-293 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MEN1, also named as Menin or MEAI, is a tumor suppressor protein encoded by the MEN1 gene. Its inactivation has been implicated in tumors of many endocrine tissues. Germline inactivating mutations of MEN1 gene are the main cause of multiple endocrine neoplasia type 1 (MEN1), a syndrome characterized by tumors in the pituitary gland, the parathyroid glands, and the enteropancreatic neuroendocrine tissues. Recent study shows astroglia menin deficiency leads to depressive-like behaviors in mice Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7253 Product name: Recombinant human MEN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 226-575 aa of BC002544 Sequence: QKLLWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPEEPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPAASPPPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL Predict reactive species Full Name: multiple endocrine neoplasia I Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 63-70 kDa GenBank Accession Number: BC002544 Gene Symbol: MEN1 Gene ID (NCBI): 4221 RRID: AB_2250607 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00255 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.