Proteintech, 15177-1-AP, Retrolinkin Polyclonal antibody

Size: 20ul / 150ul The Retrolinkin (15177-1-AP) by Proteintech is a Polyclonal antibody targeting Retrolinkin in WB, IHC, ELISA applications with reactivity to human, mouse samples 15177-1-AP targets Retrolinkin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Retrolinkin, also named as TMEM108, KIAA1690 and RTLN, is a membrane protein which plays an important role in retrograde axonal transport. It's highly enriched in neuronal endosomes, binds directly to BPAG1n4. This gene has two isoforms with calculated MW 60 kDa and 50 kDa. With modification, the MW of these two forms will be migrated to 65-70 kDa and 55-60 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7076 Product name: Recombinant human TMEM108 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 100-466 aa of BC000568 Sequence: IAATVTAPHSESSLSTGPAPAAMATTSSKPEGRPRGQAAPTILLTKPPGATSRPTTAPPRTTTRRPPRPPGSSRKGAGNSSRPVPPAPGGHSRSKEGQRGRNPSSTPLGQKRPLGKIFQIYKGNFTGSVEPEPSTLTPRTPLWGYSSSPQPQTVAATTVPSNTSWAPTTTSLGPAKDKPGLRRAAQGGGSTFTSQGGTPDATAASGAPVSPQAAPVPSQRPHHGDPQDGPSHSDSWLTVTPGTSRPLSTSSGVFTAATGPTPAAFDTSVSAPSQGIPQGASTTPQAPTHPSRVSESTISGAKEETVATLTMTDRVPSPLSTVVSTATGNFLNRLVPAGTWKPGTAGNISHVAEGDKPQHRATICLSK Predict reactive species Full Name: transmembrane protein 108 Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 55-60 kDa and 65-70 kDa GenBank Accession Number: BC000568 Gene Symbol: TMEM108 Gene ID (NCBI): 66000 RRID: AB_2204598 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6UXF1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.