Proteintech, 15206-1-AP, CRACR2A Polyclonal antibody

Size: 20ul / 150ul The CRACR2A (15206-1-AP) by Proteintech is a Polyclonal antibody targeting CRACR2A in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 15206-1-AP targets CRACR2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, Daudi cells Positive IP detected in: Jurkat cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7358 Product name: Recombinant human EFCAB4B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-395 aa of BC004524 Sequence: MAAPDGRVVSRPQRLGQGSGQGPKGSGACLHPLDSLEQKETQEQTSGQLVMLRKAQEFFQTCDAEGKGFIARKDMQRLHKELPLSLEELEDVFDALDADGNGYLTPQEFTTGFSHFFFSQNNPSQEDAGEQVAQRHEEKVYLSRGDEDLGDMGEDEEAQFRMLMDRLGAQKVLEDESDVKQLWLQLKKEEPHLLSNFEDFLTRIISQLQEAHEEKNELECALKRKIAAYDEEIQHLYEEMEQQIKSEKEQFLLKDTERFQARSQELEQKLLCKEQELEQLTQKQKRLEGQCTALHHDKHETKAENTKLKLTNQELARELERTSWELQDAQQQLESLQQEACKLHQEKEMEVYRVTESLQREKAGLLKQLDFLRCVGGHWPVLRAPPRSLGSEGPV Predict reactive species Full Name: EF-hand calcium binding domain 4B Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46 kDa, 80-90 kDa GenBank Accession Number: BC004524 Gene Symbol: CRACR2A Gene ID (NCBI): 84766 RRID: AB_2246413 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BSW2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.