Proteintech, 15268-1-AP, RPS6KB2 Polyclonal antibody

Size: 20ul / 150ul The RPS6KB2 (15268-1-AP) by Proteintech is a Polyclonal antibody targeting RPS6KB2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15268-1-AP targets RPS6KB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF7 cells, MCF-7 cells Positive IP detected in: MCF-7 cells Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RPS6KB2(ribosomal protein S6 kinase beta-2) is also named as 70 kDa ribosomal protein S6 kinase 2(P70S6K2),Serine/threonine-protein kinase 14B(STK14B) and belongs to the protein kinase superfamily. It is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. The activated enzyme phosphorylates several serine residues in the C terminus of ribosomal protein S6(PMID:9878560). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7371 Product name: Recombinant human RPS6KB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC000094 Sequence: MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHCFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDTAHTRAERNILESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTACFYLAEITLALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHEGAVTHTFCGTIEYMAPEILVRSGHNRAVDWWSLGALMYDMLTGSPPFTAENRKKTMDKIIRGKLALPPYLTPDARDLVKKFLKRNPSQRIGGGPGDAADVQRH Predict reactive species Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 2 Calculated Molecular Weight: 53 kDa Observed Molecular Weight: 54 kDa GenBank Accession Number: BC000094 Gene Symbol: RPS6KB2 Gene ID (NCBI): 6199 RRID: AB_2182250 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UBS0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.