Proteintech, 15276-1-AP, EIF1 Polyclonal antibody

Size: 20ul / 150ul The EIF1 (15276-1-AP) by Proteintech is a Polyclonal antibody targeting EIF1 in WB, IP, IHC, ELISA applications with reactivity to human samples 15276-1-AP targets EIF1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells Positive IP detected in: Jurkat cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd. Specification Tested Reactivity: human Cited Reactivity: human, monkey, hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7394 Product name: Recombinant human EIF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-113 aa of BC005118 Sequence: MSAIQNLHSFDPFADASKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLVEIGLAKDDQLKVHGF Predict reactive species Full Name: eukaryotic translation initiation factor 1 Calculated Molecular Weight: 13 kDa GenBank Accession Number: BC005118 Gene Symbol: EIF1 Gene ID (NCBI): 10209 RRID: AB_2878121 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P41567 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.