Proteintech, 15310-1-AP, ALDH2 Polyclonal antibody

Size: 20ul / 150ul The ALDH2 (15310-1-AP) by Proteintech is a Polyclonal antibody targeting ALDH2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15310-1-AP targets ALDH2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, HEK-293 cells, K-562 cells, HepG2 cells, HuH-7 cells, mouse kidney tissue, mouse liver tisue, mouse testis tissue, rat kidney tissue, rat liver tissue, rat testis tissue Positive IP detected in: HepG2 cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:1000 Background Information Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7452 Product name: Recombinant human ALDH2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 166-517 aa of BC002967 Sequence: DFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS Predict reactive species Full Name: aldehyde dehydrogenase 2 family (mitochondrial) Calculated Molecular Weight: 56 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC002967 Gene Symbol: ALDH2 Gene ID (NCBI): 217 RRID: AB_2224185 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P05091 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.