Proteintech, 15315-1-AP, RRAGD Polyclonal antibody

Size: 20ul / 150ul The RRAGD (15315-1-AP) by Proteintech is a Polyclonal antibody targeting RRAGD in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15315-1-AP targets RRAGD in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IP detected in: NIH/3T3 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Ras-related GTP-binding protein D (RRAGD) is a monomeric GTP or GDP binding protein, which plays a critical role in mediating amino acid stimulated mTOR signaling pathway, a key pathway in determination of cell growth and proliferation rate. Recently, RRAGD has been reported involving the progression of ovarian cancer and colon cancer. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7557 Product name: Recombinant human RRAGD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-249 aa of BC003088 Sequence: MEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL Predict reactive species Full Name: Ras-related GTP binding D Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46-50 kDa GenBank Accession Number: BC003088 Gene Symbol: RRAGD Gene ID (NCBI): 58528 RRID: AB_3085458 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NQL2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.