Proteintech, 15317-1-AP, SLC25A15 Polyclonal antibody

Size: 20ul / 150ul The SLC25A15 (15317-1-AP) by Proteintech is a Polyclonal antibody targeting SLC25A15 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 15317-1-AP targets SLC25A15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse liver tissue, mouse stomach tissue Positive IHC detected in: human liver cancer tissue, human skin tissue, human brain tissue, human lung tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SLC25A15 gene encodes a 301-amino-acid-long mitochondrial carrier protein termed ORC1., which catalyzes the transport of cytosolic ornithine into mitochondria in exchange for citrulline (PMID: 19242930). Some mutations in the SLC25A15 gene have been reported to break the ornithine cycle and be related with human hyperornithinemia, hyperammonemia, and homocitrullinuria syndrome (PMID: 19242930, 24721342). This antibody detected SLC25A15 at an apprarant molecular mass about 33 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7563 Product name: Recombinant human SLC25A15 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC002702 Sequence: MKSNPAIQAAIDLTAGAAGGTACVLTGQPFDTMKVKMQTFPDLYRGLTDCCLKTYSQVGFRGFYKGTSPALIANIAENSVLFMCYGFCQQVVRKVAGLDKQAKLSDLQNAAAGSFASAFAALVLCPTELVKCRLQTMYEMETSGKIAKSQNTVWSVIKSILRKDGPLGFYHGLSSTLLREVPGYFFFFGGYELSRSFFASGRSKDELGPVPLMLSGGVGGICLWLAVYPVDCIKSRIQVLSMSGKQAGFIRTFLNVVKNEGITALYSGLKPTMIRAFPANGALFLAYEYSRKLMMNQLEAY Predict reactive species Full Name: solute carrier family 25 (mitochondrial carrier; ornithine transporter) member 15 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 30-33 kDa GenBank Accession Number: BC002702 Gene Symbol: SLC25A15 Gene ID (NCBI): 10166 RRID: AB_2189758 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y619 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.