Proteintech, 15340-1-AP, RPL7A Polyclonal antibody

Size: 20ul / 150ul The RPL7A (15340-1-AP) by Proteintech is a Polyclonal antibody targeting RPL7A in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15340-1-AP targets RPL7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF7 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Ribosomal proteins are a major component of ribosomes, which catalyze protein synthesis. RPL7a, which is a component of the 60S large ribosomal subunit, has additional functions involved in cell growth and differentiation that occur via interaction with human thyroid hormone receptor (THR) and retinoic acid receptor (RAR) and in turn inhibit the activities of the two nuclear hormone receptors [PMID:21505254]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7543 Product name: Recombinant human RPL7A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-266 aa of BC005128 Sequence: MPKGKKAKGKKVAPAPAVVKKQEAKKVVNPLFEKRPKNFGIGQDIQPKRDLTRFVKWPRYIRLQRQRAILYKRLKVPPAINQFTQALDRQTATQLLKLAHKYRPETKQEKKQRLLARAEKKAAGKGDVPTKRPPVLRAGVNTVTTLVENKKAQLVVIAHDVDPIELVVFLPALCRKMGVPYCIIKGKARLGRLVHRKTCTTVAFTQVNSEDKGALAKLVEAIRTNYNDRYDEIRRHWGGNVLGPKSVARIAKLEKAKAKELATKLG Predict reactive species Full Name: ribosomal protein L7a Calculated Molecular Weight: 266 aa, 30 kDa Observed Molecular Weight: 30-32 kDa GenBank Accession Number: BC005128 Gene Symbol: RPL7A Gene ID (NCBI): 6130 RRID: AB_2254051 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62424 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.