Proteintech, 15355-1-AP, RPS27 Polyclonal antibody

Size: 20ul / 150ul The RPS27 (15355-1-AP) by Proteintech is a Polyclonal antibody targeting RPS27 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 15355-1-AP targets RPS27 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, SGC-7901 cells, Jurkat cells Positive IP detected in: SGC-7901 cells Positive IHC detected in: human endometrial cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7601 Product name: Recombinant human RPS27 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-84 aa of BC002658 Sequence: MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFRRKQH Predict reactive species Full Name: ribosomal protein S27 Calculated Molecular Weight: 9 kDa Observed Molecular Weight: 9 kDa GenBank Accession Number: BC002658 Gene Symbol: RPS27 Gene ID (NCBI): 6232 RRID: AB_2180509 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P42677 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.