Proteintech, 15378-1-AP, CES2 Polyclonal antibody

Size: 20ul / 150ul The CES2 (15378-1-AP) by Proteintech is a Polyclonal antibody targeting CES2 in WB, IHC, ELISA applications with reactivity to human, mouse samples 15378-1-AP targets CES2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: BxPC-3 cells, human liver tissue, HuH-7 cells Positive IHC detected in: mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CES2(Carboxylesterase 2) is also named as ICE and belongs to the type-B carboxylesterase/lipase family. Carboxylesterases (CESs) hydrolyze ester groups of drugs and toxins. CES2 specifically catalyzes the hydrolysis of the benzoyl group of cocaine and the acetyl groups of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. CES2 differs from another liver carboxylesterase, CES1 (114835), in drug ester substrate specificity. This protein has 2 isoforms produced by alternative splicing Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag2218 Product name: Recombinant human CES2 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 323-623 aa of BC032095 Sequence: LPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL Predict reactive species Full Name: carboxylesterase 2 (intestine, liver) Calculated Molecular Weight: 62 kDa Observed Molecular Weight: 60-70 kDa GenBank Accession Number: BC032095 Gene Symbol: CES2 Gene ID (NCBI): 8824 RRID: AB_2077501 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00748 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.