Proteintech, 15400-1-AP, SATB1 Polyclonal antibody

Size: 20ul / 150ul The SATB1 (15400-1-AP) by Proteintech is a Polyclonal antibody targeting SATB1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 15400-1-AP targets SATB1 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human brain tissue, HEK-293 cells, mouse brain tissue, mouse spleen tissue, rat brain tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human oesophagus cancer tissue, human urothelial carcinoma tissue, mouse spleen tissue, mouse thymus tissue, rat brain tissue, rat spleen tissue, rat thymus tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information Epigenetic modifications and dynamic changes in chromatin organization by organizer proteins have recently been shown to play an instrumental role in regulating cancer-promoting genes. Special AT-rich binding protein (SATB1) is a unique type of global regulator that integrates higher-order chromatin organization -withregulation of gene expression. [PMID:23076250,22998183,23121661] SATB1 is a T cell-enriched transcription factor and a chromatin organizer essential for controlling genes that participate in T-cell development and activation. It regulates gene expression by periodically anchoring matrix attachment regions to the nuclear matrix and directly recruiting chromatin-modifying factors. Depending on its posttranslational modifications, SATB1 activates or represses multiple genes.Its expression is regulated by interleukin-4 (IL4) during T helper-2(Th2) cell differentiation[PMID: 20522714]. The calculated molecular weight of SATB1 is 86 kDa, but modified SATB1 is about 100 kDa (PMID: 22879953). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7615 Product name: Recombinant human SATB1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 415-763 aa of BC001744 Sequence: PKTASQSLLVNLRAMQNFLQLPEAERDRIYQDERERSLNAASAMGPAPLISTPPSRPPQVKTATIATERNGKPENNTMNINASIYDEIQQEMKRAKVSQALFAKVAATKSQGWLCELLRWKEDPSPENRTLWENLSMIRRFLSLPQPERDAIYEQESNAVHHHGDRPPHIIHVPAEQIQQQQQQQQQQQQQQQAPPPPQPQQQPQTGPRLPPRQPTVASPAESDEENRQKTRPRTKISVEALGILQSFIQDVGLYPDEEAIQTLSAQLDLPKYTIIKFFQNQRYYLKHHGKLKDNSGLEVDVAEYKEEELLKDLEESVQDKNTNTLFSVKLEEELSVEGNTDINTDLKD Predict reactive species Full Name: SATB homeobox 1 Calculated Molecular Weight: 86 kDa Observed Molecular Weight: 86-100 kDa GenBank Accession Number: BC001744 Gene Symbol: SATB1 Gene ID (NCBI): 6304 RRID: AB_2184321 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01826 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.