Proteintech, 15407-1-AP, RBM42 Polyclonal antibody

Size: 20ul / 150ul The RBM42 (15407-1-AP) by Proteintech is a Polyclonal antibody targeting RBM42 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 15407-1-AP targets RBM42 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: BxPC-3 cells, NIH/3T3 cells, HeLa cells，HepG2 cells Positive IP detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information RBM42, also known as RNA-binding motif protein 42, is a member of the RNA-binding protein family that plays critical roles in post-transcriptional regulation of gene expression. Emerging studies highlight RBM42's involvement in pre-mRNA splicing through its association with the spliceosome complex, where it contributes to the precise removal of introns and assembly of mature mRNA. Beyond splicing, RBM42 has been implicated in cellular stress responses, cell cycle regulation, and apoptosis. Dysregulation of RBM42 is linked to developmental disorders and cancers; for instance, mutations in RBM42 are associated with neurodevelopmental abnormalities, while its overexpression or loss correlates with tumor progression in certain malignancies. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7667 Product name: Recombinant human RBM42 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 129-480 aa of BC004204 Sequence: DRSHLDSPEAREAMFLRRAAVAPQRAPILRPAFVPHVLQRADSALSSAAAGPRPMALRPPHQALVGPPLPGPPGPPMMLPPMARAPGPPLGSMAALRPPLEEPAAPRELGLGLGLGLKEKEEAVVAAAAGLEEASAAVAVGAGGAPAGPAVIGPSLPLALAMPLPEPEPLPLPLEVVRGLLPPLRIPELLSLRPRPRPPRPEPPPGLMALEVPEPLGEDKKKGKPEKLKRCIRTAAGSSWEDPSLLEWDADDFRIFCGDLGNEVNDDILARAFSRFPSFLKAKVIRDKRTGKTKGYGFVSFKDPSDYVRAMREMNGKYVGSRPIKLRKSMWKDRNLDVVRKKQKEKKKLGLR Predict reactive species Full Name: RNA binding motif protein 42 Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 65-70 kDa GenBank Accession Number: BC004204 Gene Symbol: RBM42 Gene ID (NCBI): 79171 RRID: AB_2176113 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BTD8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.