Proteintech, 15505-1-AP, KISS1R-Specific Polyclonal antibody

Size: 20ul / 150ul The KISS1R-Specific (15505-1-AP) by Proteintech is a Polyclonal antibody targeting KISS1R-Specific in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 15505-1-AP targets KISS1R-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse cerebellum tissue, MCF-7 cells, SH-SY5Y cells Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells, Hela cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information KISS1R is the receptor for metastin (kisspeptin-54, 13, 14, 10), C- terminally amidated peptides of KISS-1. KISS-1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some other carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins. Specification Tested Reactivity: human, mouse Cited Reactivity: human, rat, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: KISS1 receptor Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 75 kDa, 43-45 kDa GenBank Accession Number: NM_032551 Gene Symbol: KISS1R Gene ID (NCBI): 84634 RRID: AB_10640578 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969F8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.