Proteintech, 15578-1-AP, ALDH3A1 Polyclonal antibody

Size: 20ul / 150ul The ALDH3A1 (15578-1-AP) by Proteintech is a Polyclonal antibody targeting ALDH3A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 15578-1-AP targets ALDH3A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells Positive IP detected in: A549 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293T cells Positive FC (Intra) detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:5000-1:20000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242). This antibody is specific to ALDH3A1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, rabbit Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7977 Product name: Recombinant human ALDH3A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 105-453 aa of BC004370 Sequence: LGVVLVIGTWNYPFNLTIQPMVGAIAAGNAVVLKPSELSENMASLLATIIPQYLDKDLYPVINGGVPETTELLKERFDHILYTGSTGVGKIIMTAAAKHLTPVTLELGGKSPCYVDKNCDLDVACRRIAWGKFMNSGQTCVAPDYILCDPSIQNQIVEKLKKSLKEFYGEDAKKSRDYGRIISARHFQRVMGLIEGQKVAYGGTGDAATRYIAPTILTDVDPQSPVMQEEIFGPVLPIVCVRSLEEAIQFINQREKPLALYMFSSNDKVIKKMIAETSSGGVAANDVIVHITLHSLPFGGVGNSGMGSYHGKKSFETFSHRRSCLVRPLMNDEGLKVRYPPSPAKMTQH Predict reactive species Full Name: aldehyde dehydrogenase 3 family, memberA1 Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC004370 Gene Symbol: ALDH3A1 Gene ID (NCBI): 218 RRID: AB_2273745 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P30838 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.