Proteintech, 15627-1-AP, EXOSC5 Polyclonal antibody

Size: 20ul / 150ul The EXOSC5 (15627-1-AP) by Proteintech is a Polyclonal antibody targeting EXOSC5 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, rat samples 15627-1-AP targets EXOSC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, Raji cells, HeLa cells, K-562 cells Positive IP detected in: HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:350-1:1400 Background Information CML28 is a component of the human exosome, which is a multiprotein complex involved in the 3′ processing and degradation of RNA [PMID:12359762]. CML28 is a novel 28 kDa TAA identified by serological analysis of cDNA expression libraries (SEREX) screening of a chronic myeloid leukemia (CML) cDNA expression library. As a potential target for tumor-specific immunotherapy, CML28 is superior to other known TAAs characterized by its broader spectrum expression in tumor cells and stricter expression in normal tissues [PMID:16815301]. Specification Tested Reactivity: human, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8098 Product name: Recombinant human EXOSC5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC007742 Sequence: MEEEMHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQGDTSVLAGVYGPAEVKVSKEIFNKATLEVILRPKIGLPGVAEKSRERLIRNTCEAVVLGTLHPRTSITVVLQVVSDAGSLLACCLNAACMALVDAGVPMRALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTKGLYSDTELQQCLAAAQAASQHVFRFYRESLQRRYSKS Predict reactive species Full Name: exosome component 5 Calculated Molecular Weight: 235 aa, 25 kDa Observed Molecular Weight: 28-30 kDa GenBank Accession Number: BC007742 Gene Symbol: EXOSC5 Gene ID (NCBI): 56915 RRID: AB_2293809 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NQT4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.