Proteintech, 15642-1-AP, AID Polyclonal antibody

Size: 20ul / 150ul The AID (15642-1-AP) by Proteintech is a Polyclonal antibody targeting AID in WB, IF-P, ELISA applications with reactivity to human, mouse samples 15642-1-AP targets AID in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: MG-63 cells, Ramos cells, THP-1 cells, U2OS cells, NIH/3T3 cells Positive IF-P detected in: human tonsillitis tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information AID is essential to all three genetic alterations required for antigen-specific immunoglobulin: class switch recombination (CSR), somatic bypermutation (SHM), and gene conversion. Expression of AID is normally restricted to B cells in the GC, where SHM and CSR occur. It is likely these represent AID monomer and homodimer that western blot detected two bands in purified AID from nuclear extracts, one at 52 kDa and the other at 26 kDa. (PMID:16280388) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8136 Product name: Recombinant human AID protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC006296 Sequence: MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL Predict reactive species Full Name: activation-induced cytidine deaminase Calculated Molecular Weight: 198 aa, 24 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: BC006296 Gene Symbol: AIDA/AID Gene ID (NCBI): 57379 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9GZX7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.