Proteintech, 15673-1-AP, Collagen Type XXVII Polyclonal antibody

Size: 20ul / 150ul The Collagen Type XXVII (15673-1-AP) by Proteintech is a Polyclonal antibody targeting Collagen Type XXVII in WB, IHC, ELISA applications with reactivity to human, mouse samples 15673-1-AP targets Collagen Type XXVII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, mouse heart tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Collagen Type XXVII, also named COL27A1, it is a member of the collagen fibrillar gene family . It is located in the extrcellular matrix. The calculated molecular weight of COL27A1 is 186 kDa. This antibody can recognize the 73 kDa isoform of target. The high level of Col27a1 expression in mouse cartilage suggested the possibility that Col27a1 may be regulated in cartilage by SOX9, as are Col2a1, Col11a2, Col9a1, and Col9a2 (PMID: 15922909). Expression analyses of the mouse col27a1 gene demonstrated high expression in cartilage, the eye and ear, but also in lung and colon. It is likely that the major protein product of COL27A1, proa1(XXVII), is a component of the extracellular matrices of cartilage and these other tissues. Study of this collagen should yield insights into normal chondrogenesis, and provide clues to the pathogenesis of some chondrodysplasias and disorders of other tissues in which this gene is expressed (PMID: 12714037). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8140 Product name: Recombinant human COL27A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-329 aa of BC007696 Sequence: LSWTKAGSPAPPGVIPFQSGFIFTQRARLQAPTGTIIPAALGTELALVLSLCSHRVNHAFLFAVRSRKRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVPVLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQADTYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKPQRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKITATKIPKSLPTKPSAPSTSIVPI Predict reactive species Full Name: collagen, type XXVII, alpha 1 Calculated Molecular Weight: 1860 aa, 87 kDa Observed Molecular Weight: 68-73 kDa GenBank Accession Number: BC007696 Gene Symbol: COL27A1 Gene ID (NCBI): 85301 RRID: AB_3085469 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IZC6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.