Proteintech, 15679-1-AP, HEYL Polyclonal antibody

Size: 20ul / 150ul The HEYL (15679-1-AP) by Proteintech is a Polyclonal antibody targeting HEYL in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15679-1-AP targets HEYL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MDA-MB-453s cells, HeLa cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information HEYL, also named as Hairy/enhancer-of-split related with YRPW motif-like protein, is a 328 amino acid protein, which belongs to the HEY family. HEYL as downstream effector of Notch signaling may be required for cardiovascular development. HEYL represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8163 Product name: Recombinant human HEYL protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-328 aa of BC006087 Sequence: MKRPKEPSGSDGESDGPIDVGQEGQLSQMARPLSTPSSSQMQARKKRRGIIEKRRRDRINSSLSELRRLVPTAFEKQGSSKLEKAEVLQMTVDHLKMLHATGGTGFFDARALAVDFRSIGFRECLTEVIRYLGVLEGPSSRADPVRIRLLSHLNSYAAEMEPSPTPTGPLAFPAWPWSFFHSCPGLPALSNQLAILGRVPSPVLPGVSSPAYPIPALRTAPLRRATGIILPARRNVLPSRGASSTRRARPLERPATPVPVAPSSRAARSSHIAPLLQSSSPTPPGPTGSAAYVAVPTPNSSSPGPAGRPAGAMLYHSWVSEITEIGAF Predict reactive species Full Name: hairy/enhancer-of-split related with YRPW motif-like Calculated Molecular Weight: 328 aa, 35 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC006087 Gene Symbol: HEYL Gene ID (NCBI): 26508 RRID: AB_2878167 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NQ87 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.