Proteintech, 15753-1-AP, TRBP Polyclonal antibody

Size: 20ul / 150ul The TRBP (15753-1-AP) by Proteintech is a Polyclonal antibody targeting TRBP in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15753-1-AP targets TRBP in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, mouse liver tissue, mouse spleen tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8255 Product name: Recombinant human TRBP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC005860 Sequence: MSEEEQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGDTSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAATPVPSVVLTRSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGEAARRALQYLKIMAGSK Predict reactive species Full Name: TAR (HIV-1) RNA binding protein 2 Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 43-46 kDa GenBank Accession Number: BC005860 Gene Symbol: TRBP Gene ID (NCBI): 6895 RRID: AB_2200485 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15633 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.