Proteintech, 15767-1-AP, NQO2 Polyclonal antibody

Size: 20ul / 150ul The NQO2 (15767-1-AP) by Proteintech is a Polyclonal antibody targeting NQO2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 15767-1-AP targets NQO2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, mouse liver tissue Positive IP detected in: HeLa cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8403 Product name: Recombinant human NQO2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC006096 Sequence: MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ Predict reactive species Full Name: NAD(P)H dehydrogenase, quinone 2 Calculated Molecular Weight: 231 aa, 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: BC006096 Gene Symbol: NQO2 Gene ID (NCBI): 4835 RRID: AB_2236213 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16083 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.