Proteintech, 15776-1-AP, VARS2 Polyclonal antibody

Size: 20ul / 150ul The VARS2 (15776-1-AP) by Proteintech is a Polyclonal antibody targeting VARS2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 15776-1-AP targets VARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells Positive IP detected in: HeLa cells Positive IHC detected in: human liver cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8460 Product name: Recombinant human VARS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 168-516 aa of BC008844 Sequence: TDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLQQLMDEPPAPGSPEL Predict reactive species Full Name: valyl-tRNA synthetase 2, mitochondrial (putative) Calculated Molecular Weight: 119 kDa, 57 kDa Observed Molecular Weight: 118 kDa GenBank Accession Number: BC008844 Gene Symbol: VARS2 Gene ID (NCBI): 57176 RRID: AB_2272699 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5ST30 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.