Proteintech, 15822-1-AP, PKM2-specific Polyclonal antibody

Size: 20ul / 150ul The PKM2-specific (15822-1-AP) by Proteintech is a Polyclonal antibody targeting PKM2-specific in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15822-1-AP targets PKM2-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, 293 cell, C6 cells, HEK-293 cells, HeLa cells, N/A, Recombinant protein, HEK-293T cells, MCF-7 cells, mouse stomach tissue Positive IP detected in: A549 cells, MCF-7 cells Positive IHC detected in: human lung cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform. This antibody can detect PKM2 monomer, dimer and tetramer (PMID: 36196894). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, chicken, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: PKM pyruvate kinase, muscle Calculated Molecular Weight: 58 kDa Observed Molecular Weight: 58 kDa GenBank Accession Number: NM_002654 Gene Symbol: PKM Gene ID (NCBI): 5315 RRID: AB_1851537 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P14618 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.