Proteintech, 15938-1-AP, CNOT10 Polyclonal antibody

Size: 20ul / 150ul The CNOT10 (15938-1-AP) by Proteintech is a Polyclonal antibody targeting CNOT10 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15938-1-AP targets CNOT10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CNOT10, also named as CCR4-NOT transcription complex subunit 10, is a 744 amino acid protein, which belongs to the CNOT10 family. CNOT10 lacalizes in the cytoplasm and nucleus. CNOT10 is a component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7654 Product name: Recombinant human CNOT10 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 568-717 aa of BC002928 Sequence: NPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRAPQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLALQIIKRNQLLPAVKTHSEVRKKPVFQPVHPIQPIQMSAFTTVQRK Predict reactive species Full Name: CCR4-NOT transcription complex, subunit 10 Calculated Molecular Weight: 82 kDa Observed Molecular Weight: 82 kDa GenBank Accession Number: BC002928 Gene Symbol: CNOT10 Gene ID (NCBI): 25904 RRID: AB_2229678 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H9A5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.