Proteintech, 16008-1-AP, PYCRL Polyclonal antibody

Size: 20ul / 150ul The PYCRL (16008-1-AP) by Proteintech is a Polyclonal antibody targeting PYCRL in WB, ELISA applications with reactivity to human, mouse samples 16008-1-AP targets PYCRL in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: MDA-MB-231 cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Proline conversion into P5C is coupled to the P5C reduction into proline, catalyzed by P5C reductase. This enzyme has three isoforms: PYCR1/PYCR2, which are specific for the mitochondrial compartment and the PYCRL present in cytosol. The conversion of P5C to proline, and its transport between mitochondria and cytosol, are also coupled with glucose metabolism by the pentose phosphate pathway.(PMID: 35163433) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8716 Product name: Recombinant human PYCRL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-274 aa of BC007993 Sequence: MAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK Predict reactive species Full Name: pyrroline-5-carboxylate reductase-like Calculated Molecular Weight: 274 aa, 29 kDa Observed Molecular Weight: 29 kDa GenBank Accession Number: BC007993 Gene Symbol: PYCRL Gene ID (NCBI): 65263 RRID: AB_3669228 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q53H96 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.