Proteintech, 16014-1-AP, TRAPPC9, NIBP Polyclonal antibody

Size: 20ul / 150ul The TRAPPC9, NIBP (16014-1-AP) by Proteintech is a Polyclonal antibody targeting TRAPPC9, NIBP in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16014-1-AP targets TRAPPC9, NIBP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human kidney tissue, mouse brain tissue, mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TRAPPC9, also named KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cell differentiation and play a role in vesicular transport from the endoplasmic reticulum to the Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter. Western blotting indicates the molecular weight of TRAPPC9 is 130-140 kDa, and 250 kDa may be detected with extensive posttranslational modification of TRAPPC9. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8791 Product name: Recombinant human TRAPPC9,NIBP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 563-912 aa of BC006206 Sequence: SCQENLLQDLSDDGISVSGFPLSSPFRQVVRPRVEGKPVNPPESNKAGDYSHVKTLEAVLNFKYSGGPGHTEGYYRNLSLGLHVEVEPSVFFTRVSTLPATSTRQCHLLLDVFNSTEHELTVSTRSSEALILHAGECQRMAIQVDKFNFESFPESPGEKGQFANPKQLEEERREARGLEIHSKLGICWRIPSLKRSGEASVEGLLNQLVLEHLQLAPLQWDVLVDGQPCDREAVAACQVGDPVRLEVRLTNRSPRSVGPFALTVVPFQDHQNGVHNYDLHDTVSFVGSSTFYLDAVQPSGQSACLGALLFLYTGDFFLHIRFHEDSTSKELPPSWFCLPSVHVCALEAQA Predict reactive species Full Name: trafficking protein particle complex 9 Calculated Molecular Weight: 139 kDa Observed Molecular Weight: 128-140 kDa GenBank Accession Number: BC006206 Gene Symbol: NIBP/Trappc9 Gene ID (NCBI): 83696 RRID: AB_2256482 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96Q05 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.