Proteintech, 16086-1-AP, RPL23 Polyclonal antibody

Size: 20ul / 150ul The RPL23 (16086-1-AP) by Proteintech is a Polyclonal antibody targeting RPL23 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 16086-1-AP targets RPL23 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: BxPC-3 cells, PC-3 cells, Jurkat cells, NIH/3T3 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9120 Product name: Recombinant human RPL23 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC010114 Sequence: MSKRGRGGSSGAKFRISLGLPVGAVINCADNTGAKNLYIISVKGIKGRLNRLPAAGVGDMVMATVKKGKPELRKKVHPAVVIRQRKSYRRKDGVFLYFEDNAGVIVNNKGEMKGSAITGPVAKECADLWPRIASNAGSIA Predict reactive species Full Name: ribosomal protein L23 Calculated Molecular Weight: 140 aa, 15 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC010114 Gene Symbol: RPL23 Gene ID (NCBI): 9349 RRID: AB_2181432 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62829 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.