Proteintech, 16104-1-AP, Telethonin Polyclonal antibody

Size: 20ul / 150ul The Telethonin (16104-1-AP) by Proteintech is a Polyclonal antibody targeting Telethonin in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 16104-1-AP targets Telethonin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IHC detected in: mouse skeletal muscle tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Telethonin, also known as TCAP, is a sarcomeric protein found exclusively in striated and cardiac muscle, where it localizes to the periphery of Z discs that define the border of the sarcomere. It binds to the N-terminus of myoglobin, effectively increasing its mechanical resistance. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G(PMID: 19679566). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9014 Product name: Recombinant human TCAP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC012628 Sequence: MATSELSCEVSEENCERREAFWAEWKDLTLSTRPEEGCSLHEEDTQRHETYHQQGQCQVLVQRSPWLMMRMGILGRGLQEYQLPYQRVLPLPIFTPAKMGATKEEREDTPIQLQELLALETALGGQCVDRQEVAEITKQLPPVVPVSKPGALRRSLSRSMSQEAQRG Predict reactive species Full Name: titin-cap (telethonin) Calculated Molecular Weight: 167 aa, 19 kDa Observed Molecular Weight: 19 kDa GenBank Accession Number: BC012628 Gene Symbol: TCAP Gene ID (NCBI): 8557 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15273 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.