Proteintech, 16132-1-AP, RNASEH2A Polyclonal antibody

Size: 20ul / 150ul The RNASEH2A (16132-1-AP) by Proteintech is a Polyclonal antibody targeting RNASEH2A in WB, IHC, ELISA applications with reactivity to human samples 16132-1-AP targets RNASEH2A in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, human liver tissue, MCF-7 cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9118 Product name: Recombinant human RNASEH2A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-299 aa of BC011748 Sequence: MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL Predict reactive species Full Name: ribonuclease H2, subunit A Calculated Molecular Weight: 299 aa, 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC011748 Gene Symbol: RNASEH2A Gene ID (NCBI): 10535 RRID: AB_2269729 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75792 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.