Proteintech, 16180-1-AP, MYPN-Specific Polyclonal antibody

Size: 20ul / 150ul The MYPN-Specific (16180-1-AP) by Proteintech is a Polyclonal antibody targeting MYPN-Specific in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 16180-1-AP targets MYPN-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, L02 cells Positive IHC detected in: mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myopalladin Calculated Molecular Weight: 145 kDa Observed Molecular Weight: 145 kDa GenBank Accession Number: NM_032578 Gene Symbol: MYPN Gene ID (NCBI): 84665 RRID: AB_2146767 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86TC9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.