Proteintech, 16210-1-AP, ASS1 Polyclonal antibody

Size: 20ul / 150ul The ASS1 (16210-1-AP) by Proteintech is a Polyclonal antibody targeting ASS1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16210-1-AP targets ASS1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, rat kidney tissue, SMMC-7721 cells, Jurkat cells, mouse kidney tissue Positive IP detected in: HeLa cells Positive IHC detected in: human testis tissue, human liver tissue, human placenta tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9200 Product name: Recombinant human ASS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 149-412 aa of BC009243 Sequence: EFYNRFKGRNDLMEYAKQHGIPIPVTPKNPWSMDENLMHISYEAGILENPKNQAPPGLYTKTQDPAKAPNTPDILEIEFKKGVPVKVTNVKDGTTHQTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRGIYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIAKSQERVEGKVQVSVLKGQVYILGRESPLSLYNEELVSMNVQGDYEPTDATGFININSLRLKEYHRLQSKVTAK Predict reactive species Full Name: argininosuccinate synthetase 1 Calculated Molecular Weight: 412 aa, 47 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC009243 Gene Symbol: ASS1 Gene ID (NCBI): 445 RRID: AB_2060466 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P00966 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.