Proteintech, 16250-1-AP, PRKCDBP Polyclonal antibody

Size: 20ul / 150ul The PRKCDBP (16250-1-AP) by Proteintech is a Polyclonal antibody targeting PRKCDBP in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16250-1-AP targets PRKCDBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: L02 cells, HeLa cells, MCF-7 cells, HSC-T6 cells, mouse lung tissue, rat lung tissue Positive IP detected in: A549 cells Positive IHC detected in: human normal colonNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9209 Product name: Recombinant human PRKCDBP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC011585 Sequence: MRESALEPGPVPEAPAGGPVHAVTVVTLLEKLASMLETLRERQGGLARRQGGLAGSVRRIQSGLGALSRSHDTTSNTLAQLLAKAERVSSHANAAQERAVRRAAQVQRLEANHGLLVARGKLHVLLFKEEGEVPASAFQKAPEPLGPADQSELGPEQLEAEVGESSDEEPVESRAQRLRRTGLQKVQSLRRALSGRKGPAAPPPTPVKPPRLGPGRSAEAQPEAQPALEPTLEPEPPQDTEEDPGRPGAAEEALLQMESVA Predict reactive species Full Name: protein kinase C, delta binding protein Calculated Molecular Weight: 261 aa, 28 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC011585 Gene Symbol: PRKCDBP Gene ID (NCBI): 112464 RRID: AB_2171894 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969G5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.